Efficient in-vitro regeneration protocol in chrysanthemum (Chrysanthemum morifolium) from ray florets
Keywords:
Chrysanthemum, in-vitro, ray florets, callus induction, regeneration, proliferation.Abstract
The experiment was conducted to standardize regeneration protocol from ray florets in chrysanthemum cv. Pusa Arunodaya (TQP-
06). For callus induction, the highest callusing percent (82.67%) with a minimum number of days for callus initiation (10.87 days)
was observed in MS medium supplemented with Kinetin (10 mg/l) + NAA (1.0 mg/l) in treatment (T3). Highest shooting percent
(69.33 %) with minimum number of days for shoot initiation (6.27 days) along with maximum number of shoots per explant (5.67
shoots) in MS medium supplemented with Kinetin (5.0 mg/l) + NAA (1.0 mg/l). In shoot proliferation, the highest number of shoots
after 30 days (51.67), 60 days (72.00), and 90 days (98.67) in MS medium supplemented with Kinetin (5.0 mg/l) + NAA (1.0 mg/l).
The highest rooting percent (85.00%) with least days for root initiation (7.00 days) along with maximum roots per shoot (8.00) and
optimum root length (5.00 cm) in MS medium supplemented with IBA (0.2 mg/l). The rooted plants were successfully acclimatized
in 3-4 weeks and survived under field conditions.
Downloads
References
Dash P, Singh R P and Voss F. 2000. Retrieval of new coloured chrysanthemum through organogenesis from sectorial chimera.Curr.Sci.78: 1060–70.
Datta S K and Gupta V N. (2012). Year round cultivation of garden chrysanthemum (Chrysanthemum morifolium Ramat.) through photoperiodic response. Sci. and Cult. 78: 71–7.
Hobbie, L. J. 1998. Auxin: molecular genetic approaches in Arabidopsis. Plant Physiol.y and Biochem.36(1-2): 91-102.
Karn, R., Ranjan, J. K., Ranjan, P., Das, B., & Attri, B. L. 2022.In-vitro regeneration in long-day garlic (Allium sativum). Current Horticulture. 10(1), 37-40.
Kumar A, Prasad K V, Singh S K and Kumar S. 2012 In vitro isolation of red coloured mutant from chimeric ray florets of chrysanthemum induced by gamma-ray. Indian J. Hort. 69(4): 562–7.
Kumar, G, Sindhu, S. S., Kumar, S. and Vanlalruati, V. 2017 In vitro isolation, regeneration and purification of yellow mutant in chrysanthemum (Chrysanthemum morifolium) cv. Lalit through ray floret regeneration. Indian J. Agri.Sci., 87(7), 958-63.
Liu Z and Gao S. 2007. Micropropagation and induction of autotetraploid plants of Chrysanthemum cinerariifolium (Trev.) Vis. In Vitro Cell. Dev. Biol.. 43: 404–8.
Lone and Shah TA. 2013.Gulidaudi (Chrysanthemum) The Autumn Queen. Floriculture Today: 46-47.
Mandal A K A and Datta S K.2005.Direct somatic embryogenesis and plant regeneration from ray florets of chrysanthemum. Biol. Plant.49: 29–33.
Mandal A K A, Chakrabarty D and Datta S K. 2000. Application of in vitro techniques in mutation breeding of chrysanthemum. Plant Cell Tissue and Organ Cult.60: 33–8.
Murashige T and Skoog F 1962.A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant.15: 373–97.
Nahid J S, Saha S and Hottori K. 2007. High frequency shoot regeneration from petal explants of Chrysanthemum morifolium Ramat. in vitro. Pakistan J. Biol.Sci.10: 3 356–61.
Prasad K V, Kumar S, Raju D V S, Swarup K, Singh,] O and Patil M R. (2008). In vitro isolation, purification, rapid bulking and field establishment of a promising radio-mutant Pusa Anmol from spray Chrysanthemum cv. Ajay (No. IAEA-CN-167).
Verma A K and Prasad K V. 2019. Organogenesis and anatomical study of gamma rays induced mutant of chrysanthemum (Chrysanthemum morifolium Ramat.) from ray florets. Research Journal of Biotech Vol, 14, 3.
Waseem K, Jilani M S, Khan M S, Kiran M and Khan G. 2011.Efficient in vitro regeneration of chrysanthemum (Chrysanthemum morifolium L.) plantlets from nodal segments. African Journal of Biotech.10: 1 477–84.
Xue J P, Chang Wand Zhang A M. 2004. Studies on the technology of directly inducing regenerated plantlet from leaf of Chrysanthemum morifolium. Zhongguozhongyaozazhi= China Journal of Chinese materiamedica 29(2), 132-135.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 CURRENT HORTICULTURE
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in CURRENT HORTICULTURE is vested with the Society for Horticultural Research and Development (SHRD), which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The SHRD has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to SHRD.
